Which DNA polymerase is responsible for most DNA replication in E coli?

Which DNA polymerase is responsible for most DNA replication in E coli?

DNA polymerase III

What is the rate of polymerization in E coli?

The major replicative DNA polymerase in E. coli cells is DNA polymerase III holoenzyme (Pol III HE) able to synthesize DNA with high processivity (over 50 kb/binding event) (Yao et al., 2009) and with high speed (up to 1000 nucleotides per second).

What is the average rate of polymerisation of Deoxyribonucleotides in E coli?

On average, E. coli pol III takes ~3 ms to replicate over a dNMP in template DNA in vitro (Yao et al., 2013).

How much time does DNA replication take in E coli?

90 min

How quickly can E coli divide?

20 minutes

What are the 3 reading frames?

There are three possible reading frames in any sequence, depending on the starting point. If the first frame starts at position 1, the second frame starts at position 2, and the third frame starts at position 3.

What are the A P and E sites on the ribosome?

The A site accepts an incoming tRNA bound to an amino acid. The P site holds a tRNA that carries a growing polypeptide (the first amino acid added is methionine (Met)). The E site is where a tRNA goes after it is empty, meaning that it has transferred its polypeptide to another tRNA (which now occupies the P site).

How do you know how many reading frames you need?

To identify an open reading frame:

1. Locate a sequence corresponding to a start codon in order to determine the reading frame – this will be ATG (sense strand)
2. Read this sequence in base triplets until a stop codon is reached (TGA, TAG or TAA)

Why are there six reading frames?

The genetic code reads DNA sequences in groups of three base pairs, which means that a double-stranded DNA molecule can read in any of six possible reading frames–three in the forward direction and three in the reverse.

What happens if the reading frame is changed?

The outcome of a frameshift mutation is complete alteration of the amino acid sequence of a protein. This alteration occurs during translation because ribosomes read the mRNA strand in terms of codons, or groups of three nucleotides. These groups are called the reading frame.