Which enzyme is responsible for joining fragments during replication?

Which enzyme is responsible for joining fragments during replication?

enzyme DNA ligase

What is the name of the enzyme responsible for replicating the DNA?

DNA polymerase

What holds the DNA strands apart during replication?

A protein called Helicase breaks the Hydrogen Bonds of the two strands of DNA forming a Replication Fork. Proteins called Binding Proteins keep the two DNA strands apart preserving the Replication Fork.

Does DNA have to break apart to be copied?

DNA does not have to break apart to be copied. After DNA replication is complete, there are two new DNA molecules; one molecule has both of the original strands and one molecule has two new strands of DNA.

Where does the DNA split?

The replisome is a large protein complex that unzips and copies the DNA strands. Enzymes at the front of the complex break the chemical bonds between base pairs, creating a structure resembling a fork in the road.

Why does DNA have 2 strands?

Because the two strands of a DNA molecule have complementary base pairs, the nucleotide sequence of each strand automatically supplies the information needed to produce its partner. Before replication can occur, the length of the DNA double helix about to be copied must be unwound.

Which end does DNA polymerase bind to?

DNA polymerases add nucleotides to the 3′ end of a polynucleotide chain. The polymerase catalyzes the nucleophilic attack of the 3′-hydroxyl group terminus of the polynucleotide chain on the α-phosphate group of the nucleoside triphosphate to be added (see Figure 5.22).

Why can nucleotides only be added?

Adenine pairs with thymine, thymine with adenine, cytosine with guanine and guanine with cytosine. A primer is needed to start replication. Lagging strand is synthesised in fragments. Nucleotides cannot be added to the phosphate (5′) end because DNA polymerase can only add DNA nucleotides in a 5′ to 3′ direction.