Why is a sliding DNA clamp important in DNA replication?
A DNA clamp, also known as a sliding clamp or β-clamp, is a protein complex that serves as a processivity-promoting factor in DNA replication. As a critical component of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand.
Does not affect DNA replication?
5. Which of the following does not affect DNA replication? Explanation: The antiparallel nature of DNA and end specificity of polymerase of polymerase leads to two types of strand synthesis, leading and lagging strands. Helicase helps in the unwinding of the DNA helix.
What is the role of the clamp loading protein in replication?
Clamp loaders are pentameric ATPases of the AAA+ family that operate to ensure processive DNA replication. They do so by loading onto DNA the ring-shaped sliding clamps that tether the polymerase to the DNA.
What affects DNA replication?
Environmental factors can cause DNA damage. For example, UV light creates cyclobutane dimers (between two thymidine residues in DNA) that stall the progression of the replication machinery. Drugs or toxins are also major causes of DNA damage.
What phase does damaged DNA replication occur?
S phase
What is another role of DNA polymerase III after replication is complete Why is this important?
DNA Polymerase III, Bacterial Bacterial cells contain several distinct DNA polymerases. The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis.
Why is it more important to check for errors during DNA replication than during translation?
Why is it more important to check for errors during DNA replication than during translation? A. Proteins form without the use of any kind of genetic code. Errors in translation would affect many generations of proteins.