How should an abstract be written?
An abstract summarizes, usually in one paragraph of 300 words or less, the major aspects of the entire paper in a prescribed sequence that includes: 1) the overall purpose of the study and the research problem(s) you investigated; 2) the basic design of the study; 3) major findings or trends found as a result of your …
What is the benefit of replication in statistics?
In statistics, replication is repetition of an experiment or observation in the same or similar conditions. Replication is important because it adds information about the reliability of the conclusions or estimates to be drawn from the data.
Why are there 3 replications?
Biological replicates are different samples measured across multiple conditions, e.g., six different human samples across six arrays. Using replicates offers three major advantages: Averaging across replicates increases the precision of gene expression measurements and allows smaller changes to be detected.
What is needed to replicate a study?
A replication study involves repeating a research study, generally with different situations and different regions. Since the variables of your study are entirely different from any of other studies, who used mixed-method, it cannot be called a replication.
What is an example of a replication?
Replication is the act of reproducing or copying something, or is a copy of something. When an experiment is repeated and the results from the original are reproduced, this is an example of a replication of the original study. A copy of a Monet painting is an example of a replication.
What is replication in quantitative research?
Replication refers to researchers conducting a repeated study of a project that typically has been published in a peer-reviewed journal or book. This is not the same, however, as duplication. As such, scholars who replicate previous studies avoid plagiarism or otherwise simply rehashing previous researchers’ work.
What are the three steps of replication?
Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment. During separation, the two strands of the DNA double helix uncoil at a specific location called the origin.
What are the 3 main stages of Semiconservative replication?
DNA replication steps. There are three main steps to DNA replication: initiation, elongation, and termination. In order to fit within a cell’s nucleus, DNA is packed into tightly coiled structures called chromatin, which loosens prior to replication, allowing the cell replication machinery to access the DNA strands.
What is the process of replication?
DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Replication is an essential process because, whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell.
What are the 6 steps of DNA replication in order?
The complete process of DNA Replication involves the following steps:
- Recognition of initiation point.
- Unwinding of DNA –
- Template DNA –
- RNA Primer –
- Chain Elongation –
- Replication forks –
- Proof reading –
- Removal of RNA primer and completion of DNA strand –
What are Okazaki fragments?
Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication.
What is needed for DNA replication select all that apply?
Answer: The things needed for DNA replication are: For two identical DNA molecules to be produced, the parent DNA molecule will be unwound by an enzyme known as helicase which breaks the hydrogen bonds between the nitrogenous bases of the two DNA strands thereby separating the two strands.
What is DNA replication write down its method?
DNA replication is the process by which DNA makes a copy of itself during cell division. The separation of the two single strands of DNA creates a ‘Y’ shape called a replication ‘fork’. The two separated strands will act as templates for making the new strands of DNA.
What step of DNA replication is the most important?
2) One of the most important steps of DNA Replication is the binding of RNA Primase in the the initiation point of the 3′-5′ parent chain.
Does DNA replication occur before cell division?
DNA replication needs to occur because existing cells divide to produce new cells. So the DNA needs to be copied before cell division so that each new cell receives a full set of instructions!
Why does DNA replication occur before cell division?
In order for all of the cells in your body to maintain a full genome, each cell must replicate its DNA before it divides so that a full genome can be allotted to each of its offspring cells. If DNA replication did not take place fully, or at all, the offspring cells would be missing some or all of the genome.
What are mistakes in DNA replication called?
Mutations. Diagram of a DNA molecule looking like a twisted ladder. When there is a mistake in the copying of the genetic message that is permanent, a mutation has occurred. UV light can cause mutations, as the DNA molecules are good absorbers of UV.
What can happen if cells do not duplicate correctly?
Answer: If the cell has not properly copied its chromosomes, an enzyme called cyclin dependent kinase, or CDK, will not activate the cyclin, and the cell cycle will not proceed to the next phase. The cell will undergo cell death.
What results at the end of meiosis?
In contrast to a mitotic division, which yields two identical diploid daughter cells, the end result of meiosis is haploid daughter cells with chromosomal combinations different from those originally present in the parent. In sperm cells, four haploid gametes are produced.
What happens if cytokinesis does not occur?
Cytokinesis is a division of cytoplasm that separates a cell into two different cells. If cytokinesis did not happen, multinucleated cells would form. That is, cells would have multiple nuclei (formed from mitotic divisions) but one single cytoplasm (due to the lack of cytokinesis).
What happens if metaphase goes wrong?
If the process of mitosis goes wrong, it usually happens in a middle phase of mitosis called metaphase, in which the chromosomes move to the center of the cell and align in an area called the metaphase plate. These mutations can lead to harmful results such as cell death, organic disease or cancer.