What are the different cell culture media used for mammalian cell culture?
| Media Type | Examples | |
|---|---|---|
| Natural media | Tissue Extracts | Extract of liver, spleen, tumors, leucocytes and bone marrow, extract of the bovine embryo and chick embryo |
| Clots | coagulants or plasma clots | |
| Artificial media | Balanced salt solutions | PBS, DPBS, HBSS, EBSS |
| Basal media | MEM DMEM |
What are the media used for animal tissue culture?
Currently, animal tissue culture media are broadly categorized into four major classes: serum‐containing media, serum‐free media, protein‐free media, and chemically defined media. This chapter summarizes types, composition and different development phases of animal cell culture media.
What is a mammalian cell culture?
Mammalian cell culture is the process of growing animal cells in vitro in a flask or dish. This unit describes the methods, equipment, supplies, and reagents used in a cell culture laboratory. Cell culture media and additives are discussed.
What is complete media cell culture?
A culture medium that is enriched to contain all of the growth requirements of a strain of organisms.
What are types of culture media?
These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.
Why is phenol red used in culture media?
Phenol red is used as a pH indicator. Phenol red is used widely in culture media to identify changes from neutral to acidic pH values. It is typically used in cell culture media at 11 mg/L. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells.
What is the pH of culture media?
Apart from complete nutritional composition, right and stable pH is another important requirement for optimum microbial growth in culture media. The pH of a culture medium should be suitable to the microorganisms that will be grown. Most bacteria grow in pH 6.5 – 7.0 while most animal cells thrive in pH 7.2 – 7.4.
Is phenol red acidic or basic?
Most commonly, phenol red is an indicator dye, used as an acid pH indicator with a transition point of 7.5 (acid side to yellow / alkaline side to red)
Why is Dmem red?
DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).
What is the difference between DMEM and RPMI?
RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
What is the pH of Dmem?
7.5
What color is phenol red in basic conditions?
A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions.
What is the purpose of phenol red broth?
Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria. It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate.
What is phenol red indicator?
Phenol red, also known as phenolsulfonphthalein, is a pH indicator dye that exhibits a gradual transition from yellow to red over a pH range of 6.2 to 8.2 (Figure 2). Above 8.2 the dye turns a bright fuchsia color.
Is phenolphthalein the same as phenol red?
I hope that helps! Answer 2: Phenolpthalein is very similar to phenol red. In fact, most of the molecule is identical, except for a small group that dangles off the side of the main structure.
What is the pH of phenolphthalein?
As an indicator of a solution’s pH, phenolphthalein is colourless below pH 8.5 and attains a pink to deep red hue above pH 9.0.
How does phenol red work in a media?
Phenol red is used as a pH indicator. At low pH levels, it turns the media yellow, while at higher pH levels it turns the media purple. For most tissue culture work (pH 7.4), the media is bright red. Unfortunately, phenol red can mimic the action of some steroid hormones, particularly estrogen.
Why phenolphthalein is used in strong acid base titration?
A strong acid- strong base titration is performed using a phenolphthalein indicator. Phenolphtalein is chosen because it changes color in a pH range between 8.3 – 10. It will appear pink in basic solutions and clear in acidic solutions. Neutralization is the basis of titration.
Why phenolphthalein is a weak acid?
Phenolphthalein is a colourless, weak acid which dissociates in water forming pink anions. Under acidic conditions, the equilibrium is to the left,and the concentration of the anions is too low for the pink colour to be observed.
What is the function of phenolphthalein in titration?
Phenolphthalein is often used as an indicator in acid–base titrations. For this application, it turns colorless in acidic solutions and pink in basic solutions. It belongs to the class of dyes known as phthalein dyes.
Why is phenolphthalein pink?
Phenolphthalein, an acid-base indicator used to test the pH of a solution, turns pink due to the presence of a weak base. The indicator itself is a weak, colorless acid that forms pink anions when it dissolves in water. Although the anions are pink, the solution remains colorless in the presence of an acid.
Does the pink Colour of phenolphthalein reappear?
Phenolphthalein is basic indicator which remain colorless in acidic and neutral solution and turns pink or magenta in basic solution. (iii) If few drops of NaOH is added again to the same solution, the solution become basic and the pink color of the phenolphthalein reappears.
Why does NaOH turn pink when phenolphthalein is added?
Ionization occurs when a molecule gains or loses electrons, and this gives the molecule a negative or positive electric charge. Adding hydroxide ions (OH-, as found in bases) will change the phenolphthalein into its ion and turn the solution pink.
What Colour is phenolphthalein in neutral?
Other indicators
| Indicator | Acidic | Neutral |
|---|---|---|
| Methyl orange | Red | Yellow |
| Phenolphthalein | Colourless | Colourless |
Is phenolphthalein a synthetic indicator?
Phenolphthalein: Phenolphthalein is a synthetic indicator which is colourless in acidic medium but gives pink colour in basic medium. It is majorly used in acid – base titrations.
What are different types of indicators?
Type of indicators
- Input indicators. These indicators refer to the resources needed for the implementation of an activity or intervention.
- Process and output indicators. Process indicators refer to indicators to measure whether planned activities took place.
- Outcome indicators.
- Impact indicators.
Is phenolphthalein a universal indicator?
A universal indicator is a pH indicator made of a solution of several compounds that exhibits several smooth colour changes over a wide range pH values to indicate the acidity or alkalinity of solutions….Universal indicator.
| Indicator | Phenolphthalein |
|---|---|
| Low pH colour | Colourless |
| Transition pH range | 8.3 – 10.0 |
| High pH colour | Fuchsia |
What is the Colour of universal indicator?
When universal indicator is added to a solution, the color change can indicate the approximate pH of the solution. Acids cause universal indicator solution to change from green toward red. Bases cause universal indicator to change from green toward purple.
Why universal indicator is not suitable for titration?
Originally Answered: Why is a universal indicator not suitable for titration? There is no universsl chemical indicator. There are only chemicals that change color in solution when the pH of the solution changes in a particular narrow range. Therefore one must employ different chemical indicators at different pH ranges.
What is the Colour of universal indicator in water?
Distilled water is chemically neutral, being neither acidic nor alkaline. Universal indicator turns green-yellow in neutral solutions.