Which objective lens should you always use first when viewing a new slide?
3. When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.
Which objective lens has the highest magnification?
Lab 1 Microscopy
| Question | Answer |
|---|---|
| This objective lens provides the highest magnification | Oil immersion |
| This objective lens provides the second highest magnification | High-dry |
| This objective lens provides the lowest magnification | Low Power |
| This objectivie lens has the shortest working distance | oil immersion |
What is the relationship between field of view and total magnification?
There is an inverse relationship between the total magnification and the diameter of the field of view – i.e., as magnifications increases the diameter of the field decreases in proportion, so the diameter of field of view at different magnification can be calculated mathematically, using the formula.
What is the relationship between magnification and field of view quizlet?
The field of view is inversely proportional to the magnification of the objective lens. For example, if the diameter of your field of view is 1.78 millimeters under 10x magnification, a 40x objective will be one-fourth as wide, or about 0.45 millimeters.
Why can’t you use the largest objective for some slides?
Some slides will not focus on the largest objective because it is too close the slide will be too close to the objective lens, or because the slide shows an image that is best seen on a lower power.
What is the field of view diameter at 40x magnification?
| Objective | Diameter Of Field Of View | Magnification (10x Ocular) |
|---|---|---|
| 4x | 4.0 mm (4.45) | 40x |
| 10x | 2.0 mm (1.78) | 100x |
| 40x | 0.4 mm (0.45) | 400x |
| 100x | 0.2 mm (0.178) | 1000x |
What part of the microscope makes the image clearer?
Condenser – These are lenses that are used to collect and focus light from the illuminator into the specimen. They are found under the stage next to the diaphragm of the microscope. They play a major role in ensuring clear sharp images are produced with a high magnification of 400X and above.
What can you see at 100x magnification?
At 100x magnification you will be able to see 2mm. At 400x magnification you will be able to see 0.45mm, or 450 microns. At 1000x magnification you will be able to see 0.180mm, or 180 microns.
Which is the appropriate way to move the objectives into place?
Look at the objective lens and the stage from the side (Figure 2, at right) and turn the coarse focus knob so that the objective lens moves downward (or the stage, if it moves, goes upward). Move it as far as it will go without touching the slide!
When switching to 100x lens What should you use?
When switching to the 100x lens, what should you use? 100x lenses should be used with a few drops of immersion oil to enhance the image.
What should you do immediately after using the 100x objective lens?
Slowly rotate your 100x oil objective lens into place and adjust the fine focus until you get a crisp and clear image. When finished viewing with your 100x oil immersion lens, carefully wipe the oil from all glass surfaces using a piece of lens cleaning paper.
What is the rule for touching lenses?
Do not touch lenses with fingers, or leave liquids on objective lenses. After use, return the objective to low power, remove the slide and if necessary wipe clean the stage, then put it all back!
What is the importance of the 100x lens?
The most powerful lens of the light microscope is the 100x oil immersion objective. Because light is refracted every time it passes through a medium with a different refractive index, (air to glass or vice versa) the quality of the image is reduced with each passage.