Can I use 100x without oil?

Can I use 100x without oil?

Never use the 100x objective without using immersion oil. When you have correctly brought your slide into focus up to 40x (step 3), then: a. Turn the nosepiece half-way between the 40x and 100x objectives. Immersion oil should never be put on any other objective than the 100x!

What are the 3 rules of microscopy?

NOTES:

• Do not touch the glass part of the lenses with your fingers. Use only special lens paper to clean the lenses.
• Always keep your microscope covered when not in use.
• Always carry a microscope with both hands. Grasp the arm with one hand and place the other hand under the base for support.

Does the fine adjustment knob move the stage?

7. Focusing knobs: located on both sides of the microscope. The larger, inner coarse adjustment knob moves the stage up and down much faster and farther than the smaller, outer fine adjustment knob. When focusing under the 40X or 100X objective, ONLY use the fine adjustment, never the coarse adjustment.

What happens when you turn the fine adjustment knob away from you?

Which Way Do I Turn the Knob? If your eyepiece(s) on your microscope is built facing away from the arm of the microscope then turning the knob away from you will raise the stage and turning the knob towards you will lower the stage.

What is the difference between coarse adjustment knob and fine adjustment knob?

Coarse adjustment knob- Focuses the image under low power (usually the bigger knob) Fine adjustment knob-Sharpens the image under all powers (usually the smaller knob) Arm- supports the body tube and is used to carry the microscope.

Why must you slowly turn the fine adjustment knob?

Also, if you use the fine adjustment knob too quickly you may also run the lens into the slide and break it. Try to focus your image carefully and slowly. At this magnification, the tiniest imperceptible movements make a large difference so practice turning the fine adjustment knob as slowly as you can.

Which adjustment knob should you use on the high power?

Use ONLY the fine adjustment knob to focus on high power. NEVER USE THE COARSE FOCUS KNOB ON HIGH POWER! The high power lens should be very close to your slide when in proper focus. If you turn the coarse adjustment knob while on high power, the objective could easily break your slide.

Which is the first adjusting knob to be used?

Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Once it is in focus, you will only need to use the fine focus.