How is the inoculation of a broth tube and a slant tube different?
Which of the following is a common reason to innoculate a culture onto a slant rather than a broth? How is the inoculation of a broth tube and a slant different? Open tubes should be held at an angle instead of straight up. Why are agar slants preferred to store stock cultures compared to agar plates?
What is the difference between a broth and a slant quizlet?
Broth=dip loop into other broth; slant=streak loop across surface (zigzag); deep=use inocul.
What is slant preparation?
An agar slant tube (or simply an agar slant) is a screw-capped culture tube partly filled with an agar mix such as nutrient agar, R2A agar, or TSA (figure 1). To make it a slant tube the agar is allowed to cool with the tube laying at an angle, resulting in a large surface area for spreading a culture.
What does slant mean?
1 : to give an oblique or sloping direction to. 2 : to interpret or present in line with a special interest : angle stories slanted toward youth especially : to maliciously or dishonestly distort or falsify. slant. noun. Definition of slant (Entry 2 of 2)
What is a slant culture?
(slant kŭl’chŭr) A culture made on the slanting surface of a medium that has been solidified in a test tube inclined from the perpendicular so as to give a greater area than that of the lumen of the tube.
How could you determine if there is any contamination on your agar slant?
If some of your agar plates become contaminated, you can often tell by examining the plate how contamination took place. If the contaminants are imbedded in the agar, the contaminant was probably poured with the medium.
Why are agar slants better for stock cultures?
Why are agar slants better suited than agar plates to maintain stock cultures? Slants are better suited because they can be capped, preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
How long can bacteria survive on agar plate?
| Table 1. Approximate time bacterial cultures remain viable in different storage conditions. | ||
|---|---|---|
| Condition | Temp (°C) | Time (approx.) |
| Agar plates | 4 | 4 – 6 weeks |
| Stab cultures | 4 | 3 weeks – 1 year |
| Standard freezer | -20 | 1 – 3 years |
Why is slant culture important?
Slanting the surface of the agar gives the bacteria a greater surface area on which to grow in a test tube. Furthermore, slants are created in test tubes that can be capped, which minimizes water loss. This is important because of the high moisture content of agar media.
How do you prepare a bacterial stock culture?
After you have bacterial growth, add 500 μL of the overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Note: Make the 50% glycerol solution by diluting 100% glycerol in dH20. Note: Snap top tubes are not recommended as they can open unexpectedly at -80°C.
How do you maintain stock culture?
Stock Culture Maintenance and Storage
- Effective maintenance of stock cultures is essential for QC, method validation and research purposes.
- Repeated subculturing may eventually lead to contamination, loss of viability and genotypic/phenotypic changes.
- Freeze-drying and cryogenic storage are preferred, but may not be practical for smaller laboratories.
How do you revive ATCC culture?
For freeze dried cultures, using a single tube of the recommended media (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Use this to rehydrate the entire pellet, and transfer the entire suspension back into the broth tube and mix well.
How do you send bacterial strains?
To ship bacteria strains, I used sterile Whatman paper, dipped in bacterial culture, but I didn’t let them dry (as long as they are not dripping, it’s fine). The paper was placed in a piece of sterile Parafilm, folded tightly, then taped on a piece of cardboard, placed in an envelope, and shipped.
How do you make bacterial stab?
Procedure
- Fill airtight, autoclavable vials with rubber or teflon caps 2/3 full of stab agar.
- Inoculate with a single colony repeatedly poking the inoculating loop into the agar.
- Incubate at 37°C for 8-12 hrs until cloudy tracks of bacteria become visible.
- Seal tightly and store in the dark at room temperature (15-22°C)
How do you activate bacteria?
Bacteria use a variety of mechanisms to direct RNA polymerase to specific promoters in order to activate transcription in response to growth signals or environmental cues. Activation can be due to factors that interact at specific promoters, thereby increasing transcription directed by these promoters.
How do you revive cells?
Thaw frozen cells rapidly (< 1 minute) in a 37°C water bath. Dilute the thawed cells slowly before you incubate them, using pre-warmed growth medium. Plate thawed cells at high density to optimize recovery. Always use proper aseptic technique and work in a laminar flow hood.
What strains ATCC?
ATCC provides top-quality microbial strains needed to maintain outstanding Quality Control programs. Commercial firms specify ATCC strains as controls for rapid identification, minimum inhibitory concentration of antibiotics and antibiotic susceptibility panels. Trust ATCC for your laboratory quality control needs.
What is a type strain?
By definition, type strains are descendants of the original isolates used in species and subspecies descriptions, as defined by the Bacteriological Code [14], that exhibit all of the relevant phenotypic and genotypic properties cited in the original published taxonomic circumscriptions.
What are control strains?
Control strains include susceptible strains to monitor test performance (not for the interpretation of susceptibility), and resistant strains to confirm that the method will detect particular mechanisms of resistance, for example, Haemophilus influenzae ATCC 49247 is a β-lactamase negative, ampicillin resistant strain …
What are strains in microbiology?
A strain is a genetic variant or subtype of a microorganism (e.g., a virus, bacterium or fungus). Microbial strains can also be differentiated by their genetic makeup using metagenomic methods to maximize resolution within species.
How do you identify bacterial strains?
Bacteria are identified routinely by morphological and biochemical tests, supplemented as needed by specialized tests such as serotyping and antibiotic inhibition patterns. Newer molecular techniques permit species to be identified by their genetic sequences, sometimes directly from the clinical specimen.
What does ATCC stand for?
American Type Culture Collection
What is a pure strain?
A group of identical individuals that always produce offspring of the same phenotype when intercrossed.
What is the strongest Indica?
The Godfather OG is the most potent marijuana strain ever. This was claimed by High Times. This Indica-dominant hybrid was measured to have the highest THC content of 34%. This strain was created by California herbal remedies and is said to be a product of crossing Grandaddy Purple, Cherry Pie, and OG Kush.
What strain is 100% Indica?
Purple Kush
What strain is pure Kush?
indica
What strain is AK 47?
sativa
What is PK Kush?
Purple Kush is a pure indica strain that emerged from the Oakland area of California as the result of crossing Hindu Kush and Purple Afghani. Its aroma is subtle and earthy with sweet overtones typical of Kush varieties. Its aroma is subtle and earthy with sweet overtones typical of Kush varieties.