What are the different types of cell culture?
There are three major types of cell culture, which include:
- Primary cell culture.
- Secondary cell culture, and.
- Cell line.
What is 2D and 3D cell culture?
The reason for the growth in 3D cell culture is it’s simply a better way of representing human tissue outside the body. 2D cell cultures only exist in two dimensions. More relevant cell models — Much better biomimetic tissue models make 3D cell cultures more physiologically relevant and predictive than 2D cultures.
What are different types of media in microbiology?
These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.
What are the components of culture media?
Components of culture media:
- Water– Source of hydrogen and oxygen.
- Electrolytes– NaCl & other electrolytes.
- Peptone– Mixture of partially digested proteins (animal or vegetable).
- Meat extract, yeast extract – Protein degradation products/carbohydrates/Inorganic salts/Growth factors.
- Blood– It enriches media.
What are the two composition of culture?
As this definition suggests, there are two basic components of culture: ideas and symbols on the one hand and artifacts (material objects) on the other. The first type, called nonmaterial culture, includes the values, beliefs, symbols, and language that define a society.
What are the uses of culture media?
Culture media contain all the elements that most bacteria need for growth and are not selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory culture collections.
Why do we sterilize culture media?
When microbiological media has been made, it still has to be sterilized because of microbial contamination from air, glassware, hands, etc. Within a few hours there will be thousands of bacteria reproducing in the media so it has to be sterilized quickly before the microbes start using the nutrients up.
What is the importance of adjusting the pH of culture media?
Apart from complete nutritional composition, right and stable pH is another important requirement for optimum microbial growth in culture media. The pH of a culture medium should be suitable to the microorganisms that will be grown. Most bacteria grow in pH 6.5 – 7.0 while most animal cells thrive in pH 7.2 – 7.4.
How do you change the pH of culture media?
If you need to adjust the pH of cell culture medium, use 1 N NaOH to raise the pH or 1 N HCL to lower the pH. Be very careful to only add a drop at a time because it is very easy to over-adjust the pH. You could try using a lower concentration of acid and base.
What is the pH of agar?
5.6 to 7.5
Why is 5 CO2 used in cell culture?
5%CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.
Why is CO2 used in cell culture incubator?
A CO2 incubator is used to culture cells to provide it with the optimum temperature, moisture (sterile environment) and to maintain optimum pH. When the media contains carbonate buffer, the CO2 gas from the cylinder is let into the incubator in such a way that the pH remains constant..
Why is sodium bicarbonate used in cell culture?
Why use Sodium Bicarbonate in Cell Culture Medium? Sodium bicarbonate improves the pH control of media incubated in a 5-10% CO2 atmosphere and provides cells with carbonate ions that are essential for the metabolic functions of most cells. Additionally, this buffer is non-toxic to cells in culture.
What is the pH of phenol red?
6.2 to 8.2
What is in DMEM media?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What is DMEM F12?
DMEM/F12 Medium is a 1:1 mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium, commonly used in growing a variety of mammalian cultures. DMEM/F12 is rich and complex, containing all 21 amino acids, 10 vitamins, glucose, zinc, and iron among other components.
What is Dmem?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
Why is FBS used in cell culture?
Fetal bovine serum (FBS) is used as a growth supplement for the in vitro cell culture of eukaryotic cells. FBS contains growth factors and very low levels of antibodies, allowing for versatility in many different cell culture applications.
Is FBS and FCS the same?
There is no difference between FBS and FCS, it is just a naming preference. So FBS and FCS are the same and both fetal, but different to BS or CS which would come from a calf already born.
Why is FBS so expensive?
FBS is also the most expensive and difficult to source serum, with prices ranging from 3x – 10x more than other bovine serum products. Growing demand, coupled with a lower supply of FBS has resulted in a volatile market for US FBS and consequently prices have increased significantly in the past few years.
Why FBS is heat inactivated?
A common treatment of FBS is heat-inactivation, where FBS is heated at 56°C for 30 minutes in a water bath with occasional shaking. The purpose is to inactivate whatever components of the complement system are present in the FBS [22], and other potential unknown inhibitors of cell growth.
Can I thaw FBS at 37 C?
Do not thaw serum at temperatures above 37°C. Bottles should be agitated by a gentle swirling motion to enhance mixing and thawing.
What is the difference between BSA and FBS?
Is Bovine Serum Albumin (BSA) and Fetal Bovine Serum (FBS) the Same Thing? Nope. Fetal Bovine Serum is a commonly used serum supplement for eukaryotic cell culture. The benefit of FBS for cell culture is its lower antibody levels and higher growth factor levels.
Why does FBS inhibit trypsin?
All Answers (13) FBS contains protease inhibitors particularly α1-antitrypsin, which inhibit the trypsin activity. Even before the addition of trypsin, cells should be washed with PBS to remove any left over FBS, because this could hinder the trypsinisation process.
Does EDTA inhibit trypsin?
EDTA is added in cell suspension to prevent cell-cell and cell-matrix interactions. Indeed, EDTA can be used for cell suspension without any trypsin, this is useful if you grow the cells to work with proteins of the plasma membrane, that might get digested by trypsin.
Does PBS inhibit trypsin?
Trypsin is inactivated by the serum in your medium (more specifically, the proteinase inhibitors) you add after your trypsin treatment. Washing your cells with PBS after treatment with trypsin is therefor not needed.