What is an Enterotube used for?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
What is the Enterotube technique?
Abstract. Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification of members of the family Enterobacteriaceae, was tested and compared with the PathoTec test system in the identification of gram-negative bacilli isolated from clinical specimens.
What is the purpose of the Enterotube II Interpretation Guide?
BBL Enterotube II is a ready-to-use identification system for the identification of Enterobacteriaceae and a variety of other oxidase negative Gram negative rods.
What are the advantages of EnteroPluri-test?
EnteroPluri-Test makes possible the identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria isolated from clinical and environmental samples. The identification is based on biochemical tests performed on culture media containing specific substrates.
How is the EnteroPluri-test performed?
Lay EnteroPluri-Test with its flat surface pointing upward and, by punching the plastic film, add 3 drops of α-naphtol and 2 drops of potassium hydroxide. The reaction is positive if a red colour develops within 20 minutes. Form the 5-digit code following the instructions provided in the paragraph CODE NUMBER FORMING.
What does a negative Voges Proskauer test mean?
Result Interpretation of Voges–Proskauer (VP) Test A copper color should be considered negative. A rust color is a weak positive reaction.
Is E coli VP positive or negative?
When the VP reagents are added to MR-VP broth that has been inoculated with Escherichia coli , the media turns a copper color. This is a negative result for the VP test. When the VP reagents are added to MR-VP broth that has been inoculated with Enterobacter cloacae , the media turns red. This is a positive VP result.
What is the end product for the Voges-Proskauer test?
The basic principle for Voges-Proskauer test is to determine the ability of some microorganisms to produce a neutral end product 2,3 butanediol from glucose fermentation.
What type of metabolism is detected with the Voges-Proskauer test?
Today, Barritt’s modification of the Voges-Proskauer test is the standard procedure used to detect the presence of acetoin as a metabolic intermediate in the fermentation of glucose via the butanediol pathway.
What is the name of the test used to determine if a bacterium can produce Acetoin?
Voges–Proskauer /ˈfoʊɡəs ˈprɒskaʊ. ər/ or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria.
What does a positive MR test mean?
MR: If the tube turns red, the test is positive for mixed acid fermentation (one or more organic acids formed during the fermentation of glucose).
Are fermentation tubes selective or differential?
These media are only differential and can detect carbohydrate fermentation and gas production. The medium contains a single carbohydrate as the differential material. Carbohydrate fermentation, as in the other tests, produces acidic wastes.
Is blood agar selective or differential?
Blood agar is differential media because 3 different types of hemolysis, or lysing of red blood cells, can be seen on this plate.
Is glucose fermentation test selective or differential?
This is a differential medium. It tests an organism’s ability to ferment the sugar glucose as well as its ability to convert the end product of glycolysis, pyruvic acid into gaseous byproducts.