What media can E coli grow in?

What media can E coli grow in?

Escherichia coli is a rod-shaped gram negative bacterium normally resident in human and other mammalian colons. It can grow rapidly on minimal medium that contains a carbon compound such as glucose (which serves both as a carbon source and an energy source) and salts that supply nitrogen, phosphorus, and trace metals.

How do you identify e coli on a culture plate?

For example, growth on a MacConkey agar plate shows that E. coli are not inhibited by bile salts and crystal violet. The pink color of the bacterial growth indicates E. coli can ferment lactose and tells you that it is a gram-negative bacterium.

Can E coli be alpha hemolytic?

One known E. coli virulence factor is the potent exotoxin alpha-hemolysin (Hly). Approximately 50% of E. coli isolates causing extraintestinal infections in humans are hemolytic (6, 8, 9, 12, 28).

What type of hemolysis is E coli?

E coli is a gram-negative bacillus that grows well on commonly used media. It is lactose-fermenting and beta-hemolytic on blood agar.

What grows MacConkey Agar?

gram-negative bacterial species

Will E coli grow on MacConkey Agar?

Selective and Differential Media MacConkey agar not only selects for Gram-negative organisms by inhibiting Gram-positive organisms and yeast but also differentiates the Gram-negative organisms by lactose fermentation. Escherichia coli and other lactose ferments will produce yellow or orange colonies.

What is the purpose of MacConkey Agar?

MacConkey Agar (MAC) is a selective and differential medium designed to isolate and differentiate enterics based on their ability to ferment lactose. Bile salts and crystal violet inhibit the growth of Gram positive organisms.

What is the selective agent in MacConkey Agar?

This medium is both selective and differential. The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria. The differential ingredient is lactose.