What type of enzymes are used to sealed Okazaki DNA fragments?
The strand with the Okazaki fragments is known as the lagging strand. As synthesis proceeds, an enzyme removes the RNA primer, which is then replaced with DNA nucleotides, and the gaps between fragments are sealed by an enzyme called DNA ligase.
Which replication enzyme seals the nicks between Okazaki fragments?
| Table 1. The Molecular Machinery Involved in Bacterial DNA Replication | |
|---|---|
| Enzyme or Factor | Function |
| Ligase | Seals the gaps between the Okazaki fragments on the lagging strand to create one continuous DNA strand |
| Primase | Synthesizes RNA primers needed to start replication |
What enzyme seals the two strands of DNA together?
DNA ligase
What enzyme fills in the gaps between Okazaki fragments?
DNA polymerase
Why are leading and lagging strand primers removed rather than joined with Okazaki fragments?
Why are leading and lagging strand primers removed rather than joined with Okazaki fragments? They contain nucleotides with 2’OH groups, and are targeted for excision by DNA Polymerase. Removal of the lagging strand primer leaves a gap in the one of the strand’s DNA sequences.
What is an Okazaki fragment quizlet?
Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication. They are complementary to the lagging template strand, together forming short double-stranded DNA sections.
How does semi conservative replication work?
According to the semiconservative replication model, which is illustrated in Figure 1, the two original DNA strands (i.e., the two complementary halves of the double helix) separate during replication; each strand then serves as a template for a new DNA strand, which means that each newly synthesized double helix is a …
Why is DNA replication called a semi conservative process quizlet?
DNA replication is said to be semiconservative because each newly made DNA molecule has one original and one new strand of DNA. A laboratory technique used to replicate, and thus amplify, a specific DNA segment.
Is DNA replication describe as conservative or semi conservative why quizlet?
Terms in this set (15) DNA replication is described as semi-conservative. This means that each new DNA double-helix strand contains one strand of the original parent strand.
What is replicated by a semi-conservative process quizlet?
The semiconservative replication of DNA means that a newly formed DNA molecule has one new strand and one strand from the original DNA. If replication were a conservative process, the original DNA would have two heacy strands and all newly formed DNA woul dhave light strands.
Where does DNA replication begin quizlet?
DNA replication will begin at specific sites called origins of replication where the two DNA strands are separated opening up a replication bubble. At the end of each replication bubble is a replication fork. Which is a Y-shaped region and is the site of active replication.
Where and when specifically does DNA replication occur?
DNA replication occurs in the cytoplasm of prokaryotes and in the nucleus of eukaryotes. Regardless of where DNA replication occurs, the basic process is the same. The structure of DNA lends itself easily to DNA replication. Each side of the double helix runs in opposite (anti-parallel) directions.
Where does DNA replication occur in prokaryotes and eukaryotes?
In prokaryotic cells, there is only one point of origin, replication occurs in two opposing directions at the same time, and takes place in the cell cytoplasm. Eukaryotic cells on the other hand, have multiple points of origin, and use unidirectional replication within the nucleus of the cell.
What two processes make up the M stage?
The M phase consists of mitosis and cytokinesis. Mitosis is the process in which DNA condenses into visible chromosomes, which is followed by the separation of the chromosomes into two identical sets.
Is in between the times when a cell is dividing?
Interphase is in between the times when a cell is dividing.
What is Stage 2 of the cell cycle?
Cytokinesis, or “cell motion,” is the second main stage of the mitotic phase during which cell division is completed via the physical separation of the cytoplasmic components into two daughter cells.