Which of the following does proofreading during DNA replication?
DNA polymerases are the enzymes that build DNA in cells. During DNA replication (copying), most DNA polymerases can “check their work” with each base that they add. This process is called proofreading. Polymerase uses 3′ to 5′ exonuclease activity to remove the incorrect T from the 3′ end of the new strand.
What enzyme proofreads in DNA replication?
DNA polymerase
Is DNA replication faster in prokaryotes or eukaryotes?
Prokaryotic cells possess one or two types of polymerases, whereas eukaryotes have four or more. Replication also happens at a much faster rate in prokaryotic cells, than in eukaryotes.
Why is bromophenol blue added to the individual DNA samples?
Why is bromophenol blue added to the individual DNA samples? It allows the observer to view how far the DNA samples travel. The electrophoresis buffer is poured over the agarose gel because it charges the DNA samples so they can travel more readily across the chamber.
Why is bromophenol blue used in gel electrophoresis?
It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.
Why is detergent added during DNA isolation quizlet?
what is the liquid detergent used for in DNA isolation? it causes the cell membrane to break down, and it emulsifies the lipids and proteins of the cell by disrupting the nonpolar interactions that holds the cell membrane together. You just studied 31 terms!
Is a DNA fragment with 100 base pairs smaller than a DNA fragment with 150 base pairs?
-It helps separate the DNA bands in each sample. A DNA fragment with 100 base pairs is smaller than a DNA fragment with 150 base pairs.
How do you count base pairs in DNA?
The rules of base pairing (or nucleotide pairing) are:
- A with T: the purine adenine (A) always pairs with the pyrimidine thymine (T)
- C with G: the pyrimidine cytosine (C) always pairs with the purine guanine (G)