Which of the following enzymes are not involved in DNA replication?

Which of the following enzymes are not involved in DNA replication?

DNA polymerase I is the enzyme that replaces the RNA nucleotides in a primer with DNA nucleotides. Which of the following is not involved in the initiation of replication? Answer a. Ligase is not involved in the initiation of replication.

What is the most important step in DNA replication?

2) One of the most important steps of DNA Replication is the binding of RNA Primase in the the initiation point of the 3′-5′ parent chain. RNA Primase can attract RNA nucleotides which bind to the DNA nucleotides of the 3′-5′ strand due to the hydrogen bonds between the bases.

What is the difference between prokaryotes and eukaryotes in DNA replication?

Both prokaryotic and eukaryotic DNA undergo replication by the enzyme DNA polymerase. The main difference between prokaryotic and eukaryotic DNA is that prokaryotic DNA is found in the cytoplasm whereas eukaryotic DNA is packed into the nucleus of the cell.

How does DNA replication in prokaryotes?

DNA Replication in Prokaryotes: A replication fork is formed when helicase separates the DNA strands at the origin of replication. The DNA tends to become more highly coiled ahead of the replication fork. DNA ligase seals the gaps between the Okazaki fragments, joining the fragments into a single DNA molecule.

Is prokaryotic DNA replication unidirectional?

Bidirectional replication of DNA is the mechanism employed in all eukaryotic and most prokaryotic cells. Unidirectional replication is rare and appears to occur in only a limited number of prokaryotes.

Is DNA replication in eukaryotes bidirectional?

As with prokaryotes, DNA replication in eukaryotic cells is bidirectional. However, unlike the circular DNA in prokaryotic cells that usually has a single origin of replication, the linear DNA of a eukaryotic cell contains multiple origins of replication (Figure 19.5.

Is bacterial DNA replication Semiconservative?

Following publication of Meselson and Stahl’s results, many scientists confirmed that semiconservative replication was the rule, not just in E. coli bacteria in the presence of radioactive nucleotides such that, after replication, each new DNA molecule had one radioactive (hot) strand and one nonradioactive strand.